Secondary fluorescent staining of virus antigens by rheumatoid factor and fluorescein-conjugated anti-IgM.

IgM-rheumatoid factor and anti-cyclic citrullinated peptide decrease by 50% during intensive treatment in early rheumatoid arthritis.

calves. Based on this, we also started 50 mg/day prednisolone; a decision which would have been questionable only on the basis of our clinical findings. After 3 months the tissue tautness had improved markedly and eosinophils were normal (336 cells/mm). The first description of MRI changes in EF was made in 1989. Since then, sporadic publications of MRI features of EF have appeared mainly in ra...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Fluorescein-conjugated Bovine Albumin

Fluorescein-bovine albumin conjugates have been prepared and found not to differ appreciably in size, shape, and homogeneity from the precursor, bovine serum albumin. Fluorescein has also been conjugated to rat plasma proteins. Their disappearance rates from the circulation of rats correspond with those obtained from the use of isotope labeling. Their sites of localization in rat tissues were s...

Fluorescent anti-heart IgM and raised levels of serum IgM in newborns with congenital heart diseases.

Thirty-five per cent (13 of 37) of newborns, studied at less than 2 weeks of age, with congenital heart lesions, had abnormally high serum levels of both IgM (>20 mg/Ioo ml) and anti-heart IgM demonstrable by a fluorescent technique. An association between the raised serum IgM, reflecting in utero infection, and the congenital heart defects may be deduced. Possible explanations of the presence ...

Specific staining of wall mannan in yeast cells with fluorescein-conjugated concanavalin A.

A procedure is given for the coupling of fluorescein isothiocyanate to concanavalin A, a protein which specifically combines with a variety of polysaccharides, and for the subsequent isolation of the reactive conjugate. This fluorescent conjugate stains Saccharomyces cerevisiae but not Schizosaccharomyces pombe or Rhodotorula glutinis. The cell walls of the latter two organisms do not contain b...